Zn protoporphyrin IX is formed not from heme but from protoporphyrin IX
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چکیده
منابع مشابه
Iron protoporphyrin IX (hemin) but not tin or zinc protoporphyrin IX can stimulate gene expression in K562 cells from enhancer elements containing binding sites for NF-E2.
Many genes whose transcription is erythroid-specific contain enhancer or promoter elements that bind the transcription factor NF-E2. Hemin induction increases the expression of globin genes in the human erythroleukemia cell line K562, and increases the expression of reporters gene regulated by an enhancer elements containing binding sites for NF-E2. The failure of metalloporphyrins other than h...
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We describe a method for simultaneously measuring concentrations of coproporphyrin, zinc protoporphyrin IX, and protoporphyrin IX in whole blood by liquid chromatography, with use of reversed-phase ion-pair system, fluorometric detection, and internal standardization. Each analysis requires 10 microL of whole blood and 15 min total analysis time. Analytical recovery ranged from 84 to 92%, day-t...
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The oxidation of protoporphyrinogen IX to protoporphyrin TX in yeast cells is enzyme-dependent. The enzyme, protoporphyrinogen oxidase, associated with purified mitochondria isolated from Saccharomyces cereuisiae was solubilized by sonic treatment in the presence of detergent and partially purified. The molecular weight of the enzyme was 180,000 f 18,000. The purified preparation could be store...
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Catalase has been isolated and crystallized from the bacterium, Micrococcus lysodeikticus, but the prosthetic group of the enzyme was identified only by indirect means (I). The method described by Herbert and Pinsent (1) for the isolation of catalase from bacteria employed 78 liters of starting material. This procedure could not be applied with equal success when the starting material consisted...
متن کاملAnti-Stokes fluorescence from endogenously formed protoporphyrin IX – Implications for clinical multiphoton diagnostics
Multiphoton imaging based on two-photon excitation is making its way into the clinics, particularly for skin cancer diagnostics. It has been suggested that endogenously formed protoporphyrin IX (PpIX) induced by aminolevulinic acid or methylaminolevulinate can be applied to improve tumor contrast, in connection to imaging of tissue autofluorescence. However, previous reports are limited to cell...
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ژورنال
عنوان ژورنال: Meat Science
سال: 2007
ISSN: 0309-1740
DOI: 10.1016/j.meatsci.2007.05.008